Lemon extract

Lemon ( Citrus limon), probably had its origins in Southeast Asia and was imported into the Mediterranean regions around 300 BC.

Citrus limon, commonly known as the lemon, is a small evergreen tree or shrub native to Asia, particularly the Indian subcontinent and China. It is widely cultivated for its fruit, which is valued for its tart flavor and high vitamin C content. The lemon tree is known for its aromatic leaves and bright yellow fruit, which are used in culinary, medicinal, and cosmetic applications.

Botanical Classification:

• Kingdom: Plantae
• Order: Sapindales
• Family: Rutaceae
• Genus: Citrus
• Species: Citrus limon

Plant Characteristics:

• Growth Form: The lemon tree typically grows to a height of 3-6 meters (10-20 feet) and has a spreading canopy. It is evergreen and retains its foliage throughout the year.
• Leaves: The leaves are glossy, dark green, and have a distinctive, aromatic scent. They are ovate to elliptical, with a smooth margin and a pointed tip. The leaves are about 5-10 cm (2-4 inches) long.
• Flowers: Lemon trees produce fragrant white to pale pink flowers with a five-petaled structure. The flowers are usually solitary or in small clusters, blooming from spring to summer.
• Fruit: The fruit is a bright yellow, round to oval citrus, measuring about 6-8 cm (2.5-3 inches) in diameter. The lemon has a thick, textured rind and a sour, acidic pulp with a high vitamin C content.

Chemical Composition and Structure:

• Essential Oils: Lemon essential oil is extracted from the rind and contains compounds such as limonene, citronellol, and gamma-terpinene. These compounds contribute to the fruit's characteristic aroma and have various therapeutic properties.
• Citric Acid: The fruit is rich in citric acid, which contributes to its tart flavor and is used as a natural preservative and acidulant in food and beverages.
• Flavonoids: Lemons contain flavonoids like hesperidin and eriocitrin, which have antioxidant properties.
• Vitamins and Minerals: The fruit is an excellent source of vitamin C (ascorbic acid), as well as small amounts of B vitamins, potassium, calcium, and magnesium.

How to Cultivate It:

• Soil: Lemon trees prefer well-drained, sandy loam soils with a slightly acidic to neutral pH. They can tolerate a range of soil types as long as drainage is adequate.
• Climate: The lemon tree thrives in subtropical and tropical climates with warm temperatures and plenty of sunlight. It requires frost-free conditions and can be grown in USDA hardiness zones 9-11.
• Watering: Regular watering is essential, especially during dry periods. The soil should be kept consistently moist but not waterlogged. Drip irrigation is often used in commercial cultivation.
• Fertilization: Lemon trees benefit from balanced fertilizers with high potassium content. They should be fed during the growing season to promote healthy growth and fruit production.
• Propagation: Lemon trees are commonly propagated from seeds, grafting, or cuttings. Grafting is often used to ensure that the plant has desirable fruit characteristics and disease resistance.

Uses and Benefits:

• Culinary: Lemons are widely used in cooking and baking to add flavor and acidity. The juice, zest, and pulp are used in a variety of dishes, beverages, and desserts.
• Health Benefits: The high vitamin C content of lemons supports immune function, skin health, and antioxidant protection. Lemon juice is also used as a home remedy for digestive issues and detoxification.
• Cosmetic: Lemon essential oil is used in skincare products for its astringent and brightening properties. It is also used in hair care products to help with dandruff and shine.

Applications:

• Food and Beverage: Lemons are used in a wide range of culinary applications, including dressings, marinades, beverages (like lemonade), and as a garnish. Lemon zest and juice are also used in baking and confectionery.
• Cosmetics and Personal Care: Lemon essential oil is included in products such as cleansers, toners, shampoos, and body scrubs. Its natural astringent and antimicrobial properties are beneficial for skin and hair care.
• Household: Lemon juice and essential oil are used as natural cleaning agents due to their antibacterial and deodorizing properties.

Environmental and Safety Considerations:

• Environmental Impact: Lemon cultivation can have environmental impacts such as water usage and pesticide application. Sustainable practices and integrated pest management can help mitigate negative effects.
• Safety: Lemons are generally safe for consumption. However, lemon juice can be acidic and may cause irritation to sensitive skin or exacerbate certain conditions like acid reflux. It is advisable to use lemon products in moderation and consult with a healthcare provider if needed.

Most common species:

• Citrus Aurantifolia
• Citrus Limon
• Citrus Limon
• Medical Citrus

Studies

The chemical composition of the lemon includes mainly citric acid and limonene.
It contains flavonoids, which are extremely useful components for the immune defense of the human body and therefore to combat degenerative diseases such as cancer. In particular, Rutin, Quercetin, Neoeriocitrin (1).

It also contains a large amount of vitamin C, one of the most important antioxidants found in nature and an element of contrast to free radicals that are the cause of aging and many diseases related to the oxidation process of cells. However, the fruit that contains the highest amount of vitamin C is the kiwi.

A lemon juice, you know is a remedy against colds and flu and cooling diseases.

It also acts as a protector against rheumatoid arthritis.

Thin-skinned lemons are preferable as they have more juice.

The lemon have been recognized by scientific studies antimicrobial activity both in the form of nanoemulsions in essential oil and in the form of pure essential oil juice (2).

Among the various phenolic components present in lemon peel, Eriocitrin, for its anti-aging properties, has been the subject of specific studies in rats (3).

Lemon extract has also demonstrated antimicrobial activity against bacteria such as Pseudomonas aeruginosa and Klebsiella pneumoniae (4).

Lemon studies

References_________________________________________________________________

(1) Aliberti L, Caputo L, De Feo V, De Martino L, Nazzaro F, Souza LF. Chemical Composition and in Vitro Antimicrobial, Cytotoxic, and Central Nervous System Activities of the Essential Oils of Citrus medica L. cv. 'Liscia' and C. medica cv. 'Rugosa' Cultivated in Southern Italy. Molecules. 2016 Sep 18;21(9):1244. doi: 10.3390/molecules21091244. 

Abstract. Citrus medica cv. 'liscia' and C. medica cv. 'rugosa' are two taxa of citron, belonging to the biodiversity of South Italy, in particular of Amalfi Coast, in the Campania region. The chemical composition of the essential oils (EOs) from fruit peels of both C. medica cultivars was studied by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS) analyses. In all, 100 compounds were identified, 82 for C. medica cv. 'liscia', accounting for 91.4% of the total oil, and 88 for C. medica cv. 'rugosa', accounting for 92.0% of the total oil. Monoterpene hydrocarbons are the main constituents in both oils of C. medica cv. 'liscia' (79.1%) and C. medica cv. 'rugosa' (80.2%). In both oils, limonene (67.2%-62.8%) and camphene (8.5%-10.9%) are the main constituents. The antimicrobial activity of the EOs was assayed against some bacterial strains: Bacillus cereus (DSM 4313), Bacillus cereus (DSM 4384), Staphylococcus aureus (DSM 25693), Pseudomonas aeruginosa (ATCC 50071), and Escherichia coli (DSM 8579). Low concentrations of C. medica cv. 'rugosa' EO showed an inhibitory effect on P. aeruginosa and higher concentrations inhibited more B. cereus (4384) and E. coli than S. aureus. The cytotoxicity of the EO was evaluated against SH-SY5Y cell line. The influence of the EO on the expression of adenylate cyclase 1 (ADCY1) was also studied. The antimicrobial activity registered confirm their traditional uses as food preserving agents and led us to hypothesize the possible use of these oils as antimicrobials. The alterations in ADCY1 expression suggested a role for limonene in effects on the central nervous system.

(2)  Ledesma-Escobar CA, Priego-Capote F, Luque de Castro MD. Comparative Study of the Effect of Sample Pretreatment and Extraction on the Determination of Flavonoids from Lemon (Citrus limon). PLoS One. 2016 Jan 25;11(1):e0148056. doi: 10.1371/journal.pone.0148056.

Abstract. Background: Flavonoids have shown to exert multiple beneficial effects on human health, being also appreciated by both food and pharmaceutical industries. Citrus fruits are a key source of flavonoids, thus promoting studies to obtain them. Characteristics of these studies are the discrepancies among sample pretreatments and among extraction methods, and also the scant number of comparative studies developed so far. Objective: Evaluate the effect of both the sample pretreatment and the extraction method on the profile of flavonoids isolated from lemon. Results: Extracts from fresh, lyophilized and air-dried samples obtained by shaking extraction (SE), ultrasound-assisted extraction (USAE), microwave-assisted extraction (MAE) and superheated liquid extraction (SHLE) were analyzed by LC-QTOF MS/MS, and 32 flavonoids were tentatively identified using MS/MS information. ANOVA applied to the data from fresh and dehydrated samples and from extraction by the different methods revealed that 26 and 32 flavonoids, respectively, were significant (p≤0.01). The pairwise comparison (Tukey HSD; p≤0.01) showed that lyophilized samples are more different from fresh samples than from air-dried samples; also, principal component analysis (PCA) showed a clear discrimination among sample pretreatment strategies and suggested that such differences are mainly created by the abundance of major flavonoids. On the other hand, pairwise comparison of extraction methods revealed that USAE and MAE provided quite similar extracts, being SHLE extracts different from the other two. In this case, PCA showed a clear discrimination among extraction methods, and their position in the scores plot suggests a lower abundance of flavonoids in the extracts from SHLE. In the two PCA the loadings plots revealed a trend to forming groups according to flavonoid aglycones. Conclusions: The present study shows clear discrimination caused by both sample pretreatments and extraction methods. Under the studied conditions, liophilization provides extracts with higher amounts of flavonoids, and USAE is the best method for isolation of these compounds, followed by MAE and SE. On the contrary, the SHLE method was the less favorable to extract flavonoids from citrus owing to degradation.

(3) Yazgan H, Ozogul Y, Kuley E. Antimicrobial influence of nanoemulsified lemon essential oil and pure lemon essential oil on food-borne pathogens and fish spoilage bacteria. Int J Food Microbiol. 2019 Oct 2;306:108266. doi: 10.1016/j.ijfoodmicro.2019.108266.

(4) Shimizu C, Wakita Y, Inoue T, Hiramitsu M, Okada M, Mitani Y, Segawa S, Tsuchiya Y, Nabeshima T. Effects of lifelong intake of lemon polyphenols on aging and intestinal microbiome in the senescence-accelerated mouse prone 1 (SAMP1). Sci Rep. 2019 Mar 6;9(1):3671. doi: 10.1038/s41598-019-40253-x.

Abstract. Polyphenols have been examined for their beneficial effects on health, particularly in rodents, but their lifelong effects are unclear. Lemons (Citrus limon), containing lemon polyphenols (LPP), are widely consumed but the effects of LPP on aging are unknown. Therefore, we examined the effects of LPP on aging such as aging-related scores, locomotor activity, cognitive functions, and intestinal microbiome using senescence-accelerated mouse prone 1 (SAMP1) and senescence-accelerated resistant mouse 1 (SAMR1). All mice had ad libitum access to water (P1_water group, SAMR1) or 0.1% LPP (P1_LPP group). In the P1_LPP group, LPP intake prolonged the lifespan by approximately 3 weeks and delayed increases in aging-related scores (e.g., periophthalmic lesions) and locomotor atrophy. The P1_water group showed large changes in the intestinal microbiome structure, while the R1 and P1_LPP groups did not. The phylum Bacteroidetes/Firmicutes, which is associated with obesity, in the P1_water group was significantly lower and higher than that in the P1_LPP and R1 groups, respectively. Although the relative abundance of Lactobacillus significantly increased in both P1 groups with aging, the P1_LPP group showed a significantly lower increase than the P1_water group. Thus, lifelong intake of LPP may have anti-aging effects on both phenotypes and the intestinal environment.

(5) Liya SJ, Siddique R. Determination of Antimicrobial Activity of Some Commercial Fruit (Apple, Papaya, Lemon and Strawberry) Against Bacteria Causing Urinary Tract Infection. Eur J Microbiol Immunol (Bp). 2018 Aug 16;8(3):95-99. doi: 10.1556/1886.2018.00014. 

 Abstract. Urinary Tract Infection (UTI) is a worldwide phenomenon in modern times, in which the dependency on antibiotics for its treatment is increasing. The current study was conducted in order to find alternatives to antibiotics by investigating some commercial fruits for their antimicrobial activity. The fruits in this study included green apple (Malus domestica), papaya (Carica papaya), lemon (Citrus limon), and strawberry (Fragaria ananassa), which were used to prepare methanolic and ethanolic extracts through Soxhlet extraction technique. The extracts were used against bacteria that cause UTI, and five different strains were selected: E. coli (ATCC: 15922), E. coli (ATCC: 25922), Pseudomonas aeruginosa (ATCC: 27853), Enterococcus faecalis (ATCC: 29212), and Klebsiella pneumoniae. Antimicrobial tests of the extracts were conducted by following the agar well diffusion method, where ciprofloxacin was used as a positive control, and autoclaved distilled water was used as a negative control. Among the fruits, apple and papaya extracts did not show any zone of inhibition against any of the tested bacteria. However, both lemon and strawberry extracts showed inhibition zone against all of the mentioned bacteria. The ethanolic extracts of lemon and strawberry were more potent than their methanolic extracts. Lemon ethanolic extract showed the highest zone of inhibition against Pseudomonas aeruginosa (ATCC: 27853) (18.34 ± 0.58) and lowest one against Klebsiella pneumoniae (16.00 ± 1.00). Strawberry ethanolic extracts showed the highest zone of inhibition against Pseudomonas aeruginosa (ATCC: 27853) (16.33 ± 0.58) and the lowest one against Klebsiella pneumoniae (13.33 ± 0.58). As antibiotic resistance is paving the way for multi-drug resistant bacteria, the results of lemon and strawberry can be considered to be used as an antimicrobial agent in treating urinary tract infections.