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admin (19653 pt) 2024-Oct-17 10:35

Lactobacillus/Hippophae Rhamnoides Ferment Filtrate is an ingredient derived from the fermentation of the fruit or parts of the Hippophae rhamnoides (Sea buckthorn) plant with the probiotic bacterium Lactobacillus. This fermentation process generates a filtrate rich in bioactive compounds that combine the benefits of sea buckthorn extract with those of probiotic ferment. It is valued for its moisturizing, soothing, and skin barrier-balancing properties, making it ideal for products targeting sensitive, irritated, or stressed skin.

Chemical Composition and Structure

The ferment filtrate contains a complex blend of nutrients, including essential fatty acids, antioxidants such as vitamin C and vitamin E, and probiotics that help strengthen and rebalance the skin barrier. Fermentation enhances the bioavailability of these nutrients, allowing for better skin penetration and absorption. The filtrate is also rich in enzymes and amino acids, which play a key role in cellular renewal.

Physical Properties

Lactobacillus/Hippophae Rhamnoides Ferment Filtrate is a clear or slightly cloudy liquid, usually colorless or very pale yellow. It is water-soluble and easily incorporated into formulations such as serums, lotions, and facial masks. Its light texture allows for quick absorption without leaving the skin greasy or sticky.

Production Process

The production process involves fermenting sea buckthorn fruit or leaves with Lactobacillus bacteria. The fermentation is conducted under controlled conditions to maximize the production of bioactive compounds. The final product is then filtered to remove bacterial cells, leaving only the nutrient-rich filtrate.

  • Cultivation of Lactobacillus: Lactobacillus strains are cultivated in a specific growth medium optimized for the proliferation of beneficial bacteria. This phase is crucial to ensure adequate production of active metabolites during fermentation.

  • Preparation of Hippophae Rhamnoides: The fruits or parts of Hippophae rhamnoides are harvested and cleaned to remove dirt and impurities. This step is essential to ensure the quality of the final extract.

  • Fermentation: The fruits or parts of Hippophae rhamnoides are then added to the growth medium containing Lactobacillus. The mixture is incubated under controlled temperature and pH conditions to promote the fermentation process, during which the bacteria convert the nutrients in the fruits into beneficial metabolites.

  • Filtration: After the fermentation period, the mixture is filtered to remove undissolved solids, resulting in a liquid filtrate rich in nutrients, probiotics, and bioactive compounds derived from the fermentation.

  • Concentration (If necessary): The filtrate may be further concentrated through evaporation or other techniques to obtain a product with a higher concentration of active ingredients.

  • Quality Control and Packaging: Finally, the Lactobacillus/Hippophae rhamnoides ferment filtrate undergoes quality control checks to verify its purity, efficacy, and compliance with standards. After analysis, it is packaged for distribution and use in cosmetic products and dietary supplements.

Applications

  • Sensitive Skin Care: Due to its soothing and balancing properties, this filtrate is ideal for sensitive or irritation-prone skin. It helps strengthen the skin barrier, reducing sensitivity and protecting the skin from external aggressors.

  • Moisturizing Products: The filtrate is a powerful moisturizer, improving skin hydration and keeping it soft and smooth. It is often used in serums and creams for dry or dehydrated skin.

  • Regenerative Products: With enzymes and amino acids, the filtrate promotes cellular regeneration and can help repair stressed or damaged skin.

INCI Functions:

Hair conditioning agent. A significant number of ingredients with specific and targeted purposes may co-exist in hair shampoo formulations: cleansers, conditioners, thickeners, matting agents, sequestering agents, fragrances, preservatives, special additives. However, the indispensable ingredients are the cleansers and conditioners as they are necessary and sufficient for hair cleansing and manageability. The others act as commercial and non-essential auxiliaries such as: appearance, fragrance, colouring, etc. Hair conditioning agents have the task of increasing shine, manageability and volume, and reducing static electricity, especially after treatments such as colouring, ironing, waving, drying and brushing. They are, in practice, dispersants that may contain cationic surfactants, thickeners, emollients, polymers. The typology of hair conditioning agents includes: intensive conditioners, instant conditioners, thickening conditioners, drying conditioners. They can perform their task generally accompanied by other different ingredients.

Health and Safety Considerations

Safety in Use
Lactobacillus/Hippophae Rhamnoides Ferment Filtrate is considered safe for use in cosmetics. It is well tolerated by the skin and does not pose significant risks of irritation or sensitization. Major regulatory authorities, such as the European Union and the FDA, approve its use in skincare products.

Allergic Reactions
Allergic reactions to this filtrate are rare, but as with all probiotic or fermented products, it is recommended to perform a patch test before use, especially on sensitive skin.

Toxicity and Carcinogenicity
It is considered a safe and beneficial ingredient for the skin, thanks to its balancing and regenerative properties.

Environmental Considerations
The ferment filtrate is sustainably produced through controlled fermentation, a low-impact environmental process. The use of probiotics and natural ingredients such as sea buckthorn makes it an eco-friendly ingredient.

Regulatory Status
Lactobacillus/Hippophae Rhamnoides Ferment Filtrate is approved for use in cosmetics by major regulatory authorities, including the European Union and the FDA in the United States. It is widely used in products.


Sea buckthorn (Hippophae rhamnoides) Studies

From the leaves and berries of the sea buckthorn (Hippophae rhamnoides) is extracted chloroform and n-hexane, components with intense antibacterial activity (1).

It is a rich source of vitamins C and E, carotenoids, flavonoids, anthocyanins, organic acids, amino acids, sterols, triterpenols and isoprenols (2) with an antioxidant activity.

The berries produce an oil containing carotenoids, palmitic, palmitolic and oleic acid, while the seed oil is composed of linoleic, linolenic and oleic acid as main components (3) .

This oil has traditionally been used to improve blood circulation and for the treatment of dermatological problems including wounds, gastritis, peptic ulcers, uterine erosion and inflammatory disorders of the genital organs (4).

Sea buckthorn has significant cardioprotective activity and a positive effects on the healing of the cardiovascular system, including inhibition of platelet activation (in particular platelet aggregation), reduction of cholesterol concentration, blood pressure and antioxidant action (5).

In this study, a positive evaluation of the topical effects on atopic lesions as it improved the severity of dermatitis by reducing epidermal thickness (6).

The results of this study on the extract of Sea buckthorn, provide a basis for affordable, low side-effect therapy for the treatment of psoriasis (7).

Cosmetics

It produces a regenerating effect on the skin produced by carotenoids, vitamin E and unsaturated fats contained in the pulp and seeds.

Other applications

It is placed in foods, candies, to give a particular taste.

Sea buckthorn studies

References_______________________________________________________________________

(1) Qadir MI, Abbas K, Younus A, Shaikh RS. Report - Antibacterial activity of sea buckthorn (Hippophae rhamnoides L.) against methicillin resistant Staphylococcus aureus (MRSA). Pak J Pharm Sci. 2016 Sep;29(5):1711-1713. 

Abstract. Objective of the present study was to investigate the antibacterial activity of Sea buckthorn (Hippophae rhamnoides L.) berries and leaves against methicillin resistant Staphylococcus aureus (MRSA) by using the standard disc diffusion method. Chloroform, n-hexane and aqueous extract of the plant parts were used. Doses of 2mg/ml, 4 mg/ml and 6mg/ml were tested against the microorganism, and the zone of inhibition was compared against the standard drug vancomycin. Results indicated that n-hexane and chloroform extracts of berries and n-hexane extract leaves showed significant (p<0.05) antibacterial activity comparable with vancomycin. It was concluded from the study that extracts berries and leaves of Hippophae rhamnoides have antibacterial activity against MRSA.

(2) Yang B, Kallio H. Supercritical CO2-extracted sea buckthorn (Hippophae rhamnoides) oils as new food ingredients for cardiovascular health. In: Proceedings of Health Ingredients of Europe, Paris; 2002, p. 17–19.

(3) Süleyman, Z., Erdemoğlu, N., Küsmenoğlu, Ş., Gürbüz, İ., Yesilada, E., and Çalış, İ. Fatty acid composition and anti-ulcerogenic activity of Hippophae rhamnoides fruit oil. J Fac Pharm Gazi Univ. 1998; 15: 11–17

(4) Li, T.S.C. and Wang, L.C.H. Physiological components and health effects of ginseng, echinacea and sea buckthorn. in: G. Mazza (Ed.) Functional foods, biochemical and processing aspects. Technomic Publishing Company, Lancaster, PA; 1998: 329–356

Abstract. Background: Increased consumer´s interest in health has driven the development of foods that offer specific beneficial effects. The list of foods and ingredients includes essential and non-essential nutrients, plant and marine components, whole foods, microorganisms, microalgae and technological approaches. Traditionally, health outcomes focussed on the prevention of chronic diseases but health targets have expanded to cover areas such as brain health, inflammation, eye health, women´s health, healthy ageing and beauty. Objective: This review highlights, from a nutritional biochemistry perspective, differential aspects on designing and interpreting human studies to support the health effects of functional foods. Results: Despite the available evidence from in vitro, animal and observational studies, welldesigned human studies are necessary to support the health effects of functional foods. Intervention trials with foods are complex as they imply limitations due to methodological, food-related and host-related factors. The use of responsive, validated and clinically relevant markers becomes essential even though there is a lack of reliable biomarkers of exposure for many bioactives. Furthermore, the effect of modulating factors such as subclinical inflammation, gut microbiota and genetic variability should be taken into account. Multiple indicators may provide a more reliable alternative to assess physiological processes while emerging biomarkers (microRNAs, epigenetic changes) constitute a promising approach. Additionally, the magnitude of the change is critical to support any health effect although interventions may have a limited clinical impact but be epidemiologically relevant. Also, based on the available data, the premise that bioactivescontaining foods are safe may be questionable. Conclusion: An integrated approach including multiple biomarkers, genetic variability, effect of gut microbiota and risk/benefit assessment should be used to support the potential health effects of functional foods.

(5) Olas B. Sea buckthorn as a source of important bioactive compounds in cardiovascular diseases. Food Chem Toxicol. 2016 Nov;97:199-204. doi: 10.1016/j.fct.2016.09.008. Epub 2016 Sep 9. PMID: 27616182.

(6) Hou DD, Di ZH, Qi RQ, Wang HX, Zheng S, Hong YX, Guo H, Chen HD, Gao XH. Sea Buckthorn (Hippophaë rhamnoides L.) Oil Improves Atopic Dermatitis-Like Skin Lesions via Inhibition of NF-κB and STAT1 Activation. Skin Pharmacol Physiol. 2017;30(5):268-276. doi: 10.1159/000479528. Epub 2017 Sep 6. PMID: 28873377.

(7) Boca AN, Ilies RF, Saccomanno J, Pop R, Vesa S, Tataru AD, Buzoianu AD. Sea buckthorn extract in the treatment of psoriasis. Exp Ther Med. 2019 Feb;17(2):1020-1023. doi: 10.3892/etm.2018.6983.

Abstract. Psoriasis is one of the most common chronic dermatological conditions, with a strong impact on patients' quality of life. Currently, psoriasis benefits from conventional therapy with a high rate of adverse effects and an increase in non-compliance and self-medication of patients. As such, there is a need to pinpoint low-adverse effects and accessible remedies for this condition. Our single-blind, placebo-controlled study assessed the effect of sea buckthorn extract on psoriasis lesions in previously untreated patients. Our results showed an improvement in Psoriasis Area Severity Index (PASI) scores and in Dermatology Life Quality Index (DLQI) scores when compared to the baseline values, as well as at the 4- and 8-week time marks for the lesions treated with sea buckthorn extract. By contrast, the measurements for the placebo treated lesions showed no alteration at the 4-week mark, and significant worsening at the end of the trial. These findings provide a solid, optimistic base for the in-depth research of sea buckthorn as an adjuvant or a component in psoriasis care protocols.