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References__________________________________________________________________________

Sanfilippo R, Timpanaro N, Canale M, Moscaritolo S, Amenta M, Allegra M, Papa M, Spina A. The Potential of Ancient Sicilian Tetraploid Wheat in High-Quality Pasta Production: Rheological, Technological, Biochemical, and Sensory Insights. Foods. 2025 Jun 11;14(12):2050. doi: 10.3390/foods14122050. 

Abstract. This study evaluated the potential of three ancient Sicilian tetraploid wheat genotypes-'Margherito', 'Perciasacchi', and 'Russello'-for organic pasta production, compared to the national variety 'Cappelli'. Significant variations in particle size distribution were found, with 'Russello' exhibiting the highest proportion of fine particles and the greatest protein content (14.30% d.m.). 'Perciasacchi' displayed the highest gluten index (81.26%). 'Margherito' and 'Cappelli' had the highest antioxidant activity, with 'Margherito' showing elevated levels of lutein and total carotenoids. Rheological analysis revealed differences in dough properties. 'Perciasacchi' exhibited the highest dough stability and P/L ratio (6.57), whereas 'Russello' showed the lowest values for both. Additionally, 'Russello' had lower consistency (12 B.U.), reduced gel stability, and limited water retention in the visco-amylographic analysis. Pasta quality was evaluated based on cooking time, water absorption, and texture. Cooking time ranged from 10 to 12 min, with 'Russello' and 'Margherito' showing lower water absorption. Texture analysis indicated that 'Margherito' pasta was the least firm, while 'Russello' showed the greatest loss of consistency when overcooked. From a sensory perspective, 'Russello' had lower firmness, but a stronger semolina flavor and surface roughness. 'Cappelli' had the most intense cooked pasta odor, while 'Perciasacchi' was the hardest and least sticky, though less flavorful. The results support the use of ancient tetraploid wheat genotypes as valuable resources for sustainable, high-quality pasta production.

Pandolfo A, Messina B, Russo G. Evaluation of Glycemic Index of Six Different Samples of Commercial and Experimental Pasta Differing in Wheat Varieties and Production Processes. Foods. 2021 Sep 18;10(9):2221. doi: 10.3390/foods10092221.

Abstract. Pasta is a staple food of the Mediterranean Diet, and it is traditionally made of durum wheat semolina. In Sicily, durum wheat production and its transformation into semolina, bread, and pasta are well-developed economic sectors. For pasta, there is a wide supply of commercial brands, whether coming from conventional industrial manufacturing or from medium to small and local handcrafted production. Both conventional durum wheat and local durum wheat landraces, such as Timilia and Russello, are used for pasta production, but local landraces are, for the most, transformed into handcrafted pasta. The market of local landraces durum wheat pasta has risen in recent decades, in Sicily and in Italy as well, boosted by a perceived high nutritional and healthy value of these wheat derivatives. In particular, a popular and scientifically unproven idea suggests that a reduced glycemic response might be elicited by these pasta landraces. Therefore, to test this hypothesis, the main objective of the present study was the evaluation of the glycemic index (GI) of four samples of Timilia and Russello handcrafted pasta and two samples of conventional durum wheat pasta. The study enrolled fourteen healthy weight male and female volunteers aged from 18 to 46; eight test sessions were performed twice a week, every session testing a pasta sample (six sessions) or the glucose solution chosen as reference food (two sessions). The standard methodology for GI measurement was followed during each step of the study. The six tested pasta samples were characterized regarding their composition (protein, fiber, and starch content) and their whole production processes (milling method and milling diagram of flour or semolina, drying temperature, and diagram of pasta shape). The six tested pasta samples showed GI values ranging from low (34.1) to intermediate (63.1). Timilia and Russello pasta are the first GI calculations available. The two samples made of conventional grains showed lower values of GI (34.1 and 37.8). The results do not support the popular idea of a reduced glycemic response elicited by Timilia and Russello wheat landrace pasta; the tested samples showed GI values in the range of 56.2 to 63.1. However, some consideration should be made of factors other than wheat varieties and related to production processes that may have affected the final GIs of the pastas. Even if the study is not designed to discriminate among factors related to wheat varieties or processes used to produce different pasta, it is a preliminary step in the characterization of the healthy potential of the local wheat landraces, popularly called ancient grain. A future implementation of the local wheat landraces supply chain should pay attention to all the factors above, from a better seed identity certification to the production process in order to further improve the healthy value of these staples of the Mediterranean Diet.

Di Francesco A, Cunsolo V, Saletti R, Svensson B, Muccilli V, De Vita P, Foti S. Quantitative Label-Free Comparison of the Metabolic Protein Fraction in Old and Modern Italian Wheat Genotypes by a Shotgun Approach. Molecules. 2021 Apr 29;26(9):2596. doi: 10.3390/molecules26092596.

Abstract. Wheat represents one of the most important cereals for mankind. However, since wheat proteins are also the causative agent of several adverse reactions, during the last decades, consumers have shown an increasing interest in the old wheat genotypes, which are generally perceived as more "natural" and healthier than the modern ones. Comparison of nutritional value for modern and old wheat genotypes is still controversial, and to evaluate the real impact of these foods on human health comparative experiments involving old and modern genotypes are desirable. The nutritional quality of grain is correlated with its proteomic composition that depends on the interplay between the genetic characteristics of the plant and external factors related to the environment. We report here the label-free shotgun quantitative comparison of the metabolic protein fractions of two old Sicilian landraces (Russello and Timilia) and the modern variety Simeto, from the 2010-2011 and 2011-2012 growing seasons. The overall results show that Timilia presents the major differences with respect to the other two genotypes investigated. These differences may be related to different defense mechanisms and some other peculiar properties of these genotypes. On the other hand, our results confirm previous results leading to the conclusion that with respect to a nutritional value evaluation, there is a substantial equivalence between old and modern wheat genotypes. Data are available via ProteomeXchange with identifier .