Hippophae Rhamnoides Callus Culture Extract is an innovative botanical ingredient derived from the cultured callus tissue of the Hippophae rhamnoides (sea buckthorn) plant. This extract is obtained through biotechnological methods that cultivate plant stem cells in controlled environments. Rich in bioactive compounds, it is particularly valued for its antioxidant function, which helps to protect the skin from oxidative stress and environmental damage. It also contains a blend of essential fatty acids, vitamins, and phytonutrients, making it a versatile ingredient in skincare formulations.
Chemical Composition and Structure
This extract is a concentrated source of antioxidants, including polyphenols, flavonoids, and carotenoids. These compounds are known for their ability to neutralize free radicals, thereby preventing cellular damage caused by oxidative stress. Additionally, the extract contains omega fatty acids (including omega-3 and omega-6), vitamins (such as vitamin C and vitamin E), and other bioactive substances that contribute to skin nourishment, repair, and protection.
Physical Properties
It typically appears as a clear or slightly viscous liquid, depending on its concentration and formulation. It is water-soluble and is often incorporated into serums, creams, lotions, and other skincare products. Its lightweight texture allows for easy absorption without leaving a greasy residue, making it ideal for daily use.
Production Process
Hippophae rhamnoides callus culture extract is produced using biotechnological processes. The callus tissue from the sea buckthorn plant is cultured in vitro in a controlled environment, allowing the extraction of highly concentrated bioactive compounds. This method not only ensures sustainability but also enhances the potency and purity of the extract, which is then refined and stabilized for use in cosmetic formulations.
Preparation of Callus Culture: The cultivation of callus occurs in a laboratory setting, using plant cells from Hippophae rhamnoides. The cells are selected and placed in a nutrient-rich growth medium, where they are stimulated to form callus through an in vitro culture process.
Incubation: The callus cells are incubated under controlled temperature and light conditions to promote growth and proliferation. During this process, the callus develops a high concentration of bioactive compounds.
Harvesting the Callus: Once the callus has reached a sufficient mass, it is harvested. This plant material is rich in nutrients and beneficial compounds.
Extraction: The extraction of active principles from the callus occurs using appropriate solvents, such as water, ethanol, or methanol. The harvested callus is immersed in the solvent, and the mixture is agitated or heated to facilitate the release of bioactive compounds.
Filtration: After extraction, the obtained solution is filtered to remove undissolved solids, resulting in a liquid extract containing the active principles of the callus culture.
Concentration: The extract may be further concentrated by evaporating the solvent to obtain a product richer in nutrients and with greater stability.
Quality Control and Packaging: Finally, Hippophae rhamnoides callus culture extract undergoes quality control checks to verify its purity, efficacy, and compliance with standards. After analysis, it is packaged for distribution and use in cosmetic products and dietary supplements.
Applications
Antioxidant Skincare: The powerful antioxidant properties of this extract make it an excellent ingredient for protecting the skin from environmental stressors, such as UV radiation and pollution. It helps to prevent premature aging by reducing the impact of oxidative stress on the skin.
Anti-Aging Products: Due to its ability to neutralize free radicals and support cell regeneration, Hippophae rhamnoides callus culture extract is widely used in anti-aging creams, serums, and treatments. It reduces the appearance of fine lines and wrinkles while improving skin elasticity and firmness.
Regenerative and Soothing Products: The extract’s blend of omega fatty acids and vitamins also makes it ideal for formulations that target skin repair and hydration. It helps soothe irritated or damaged skin, making it suitable for sensitive skin types.
INCI Functions:
Antioxidant agent. Ingredient that counteracts oxidative stress and prevents cell damage. Free radicals, pathological inflammatory processes, reactive nitrogen species and reactive oxygen species are responsible for the ageing process and many diseases caused by oxidation.
CAS: 90106-68-6 290-292-8
Health and Safety Considerations
Safety in Use
Hippophae rhamnoides callus culture extract is considered safe for use in cosmetic products. It is well tolerated by the skin and does not pose significant risks of irritation or sensitization. Major regulatory bodies, such as the European Union and the FDA, approve its use in skincare products.
Allergic Reactions
Allergic reactions to this extract are uncommon. However, as with any new skincare ingredient, it is recommended to perform a patch test prior to use, especially for those with sensitive skin or known allergies to botanical extracts.
Toxicity and Carcinogenicity
Its antioxidant properties help protect the skin from oxidative stress, which can contribute to cellular damage and skin aging.
Environmental Considerations
Hippophae rhamnoides callus culture extract is produced sustainably through biotechnological cultivation. By using plant cell cultures, it minimizes the need for large-scale harvesting of natural resources, making it an eco-friendly and renewable ingredient.
Regulatory Status
The extract is approved for use in cosmetic products by major regulatory authorities, including the European Union and the FDA in the United States. Its potent antioxidant and regenerative properties make it a sought-after ingredient in skincare formulations.
Sea buckthorn (Hippophae rhamnoides) Studies
From the leaves and berries of the sea buckthorn (Hippophae rhamnoides) is extracted chloroform and n-hexane, components with intense antibacterial activity (1).
It is a rich source of vitamins C and E, carotenoids, flavonoids, anthocyanins, organic acids, amino acids, sterols, triterpenols and isoprenols (2) with an antioxidant activity.
The berries produce an oil containing carotenoids, palmitic, palmitolic and oleic acid, while the seed oil is composed of linoleic, linolenic and oleic acid as main components (3) .
This oil has traditionally been used to improve blood circulation and for the treatment of dermatological problems including wounds, gastritis, peptic ulcers, uterine erosion and inflammatory disorders of the genital organs (4).
Sea buckthorn has significant cardioprotective activity and a positive effects on the healing of the cardiovascular system, including inhibition of platelet activation (in particular platelet aggregation), reduction of cholesterol concentration, blood pressure and antioxidant action (5).
In this study, a positive evaluation of the topical effects on atopic lesions as it improved the severity of dermatitis by reducing epidermal thickness (6).
The results of this study on the extract of Sea buckthorn, provide a basis for affordable, low side-effect therapy for the treatment of psoriasis (7).
Cosmetics
It produces a regenerating effect on the skin produced by carotenoids, vitamin E and unsaturated fats contained in the pulp and seeds.
Other applications
It is placed in foods, candies, to give a particular taste.
Sea buckthorn studies
