Whole Milk Yogurt: extended scientific profile (composition, microbiology, processing, nutrition, digestibility, regulation, and sustainability)
Definition
Whole milk yogurt is a fermented dairy product derived from whole bovine milk through controlled microbial fermentation, primarily involving thermophilic lactic acid bacteria. The end product contains live cultures, partially hydrolyzed milk proteins, and residual lactose, within a semi-solid matrix formed by acid-induced coagulation of casein micelles.
1. Composition and molecular characteristics (per 100 g, average)
Water: 85–88 g
Lipids: 3.5–4.0 g
primarily saturated fatty acids (e.g. palmitic acid, stearic acid)
short-chain fatty acids (e.g. butyric acid) contribute to rapid energy release
Proteins: 3.3–4.2 g
~80% caseins (αs1-, αs2-, β-, κ-caseins)
~20% whey proteins (β-lactoglobulin, α-lactalbumin, immunoglobulins)
Carbohydrates: 4.5–5.2 g
Minerals:
Vitamins:
2. Microbial profile
Standard yogurt fermentation is conducted with:
These bacteria exhibit proto-cooperation, with S. thermophilus producing formic acid and CO₂ to support L. bulgaricus, which in turn releases amino acids that benefit S. thermophilus.
Some formulations may include probiotic strains (e.g. L. acidophilus, Bifidobacterium animalis) to enhance functional properties.
3. Manufacturing process (industrial scale)
Standardization
Whole milk is standardized to ~3.5% fat. Total solids may be adjusted by adding milk powder or concentrating the milk.
Homogenization
Applied at ~60–70 °C and 150–250 bar to prevent cream separation and improve texture.
Pasteurization
High-heat treatment (85–90 °C for 30–45 minutes) denatures whey proteins, especially β-lactoglobulin, allowing interaction with caseins.
Cooling and inoculation
Milk is cooled to 42–45 °C, then inoculated with starter cultures (2–3% inoculum).
Fermentation
Conducted at 42–43 °C until pH drops to ~4.5 (4–6 hours), forming a gel-like network.
Post-processing
For set yogurt: directly cooled in cups.
For stirred yogurt: gel is broken, homogenized, then cooled and packed.
4. Functional health implications
Probiotic potential: if not pasteurized post-fermentation, contains viable cultures beneficial for gut microbiota
Lactose hydrolysis: fermentation reduces lactose content, enhancing digestibility for mildly intolerant individuals
Calcium bioavailability: acidic pH maintains calcium solubility, improving absorption
Immune modulation: some studies associate live cultures with immune response regulation and anti-inflammatory activity
5. Digestibility and metabolic aspects
Proteins are partially hydrolyzed during fermentation, reducing allergenicity and improving amino acid bioaccessibility
Lipid digestion is efficient due to the presence of short- and medium-chain fatty acids
Lactose is transformed into lactic acid, improving digestive tolerance
Live bacteria may aid in vitamin B12 and K2 synthesis (strain-dependent)
6. Regulatory standards (EU and Codex Alimentarius)
Must be produced with only milk and approved starter cultures
Must contain at least 10⁷ CFU/g of live bacteria at end of shelf life
No post-fermentation heat treatment allowed if labeled "live cultures" or "with probiotics"
Maximum permitted additives: none in traditional yogurt; thickeners or stabilizers allowed in flavored types (e.g., pectin, starch)
7. Sensory and technological qualities
Texture: gel-like, smooth, creamy
Aroma: mild, slightly acidic, with acetaldehyde as a key aroma compound
Taste: balanced acidity, full dairy mouthfeel due to fat content
Common defects: whey separation (syneresis), excessive sourness, gas formation (contamination)
8. Environmental considerations
Environmental impact depends on:
livestock management (feed, methane emissions, water use)
energy consumption during heating and cooling phases
packaging: commonly plastic (PP, PS, PET), with increasing interest in biodegradable and recyclable options
Sustainable dairy chains: choosing organic, pasture-fed, or local farms reduces CO₂ footprint
9. Conclusion
Whole milk yogurt is a nutrient-rich fermented food with strong health, functional, and sensory attributes. It combines a naturally balanced macronutrient profile with the benefits of live microbial cultures, contributing to digestive wellness and bone health.
From both a nutritional and technological perspective, it is superior in mouthfeel and richness compared to low-fat alternatives.
It remains a key element of the Mediterranean and global diet, with expanding interest in its probiotic, organic, and functional versions.
References__________________________________________________________________________
Fiore G, Di Profio E, Sculati M, Verduci E, Zuccotti GV. Health effects of yogurt consumption during paediatric age: a narrative review. Int J Food Sci Nutr. 2022 Sep;73(6):738-759. doi: 10.1080/09637486.2022.2065467.
Abstract. Yogurt is a fermented milk product characterised by a peculiar nutritional composition with live and viable cultures of bacteria. Few studies have analysed the benefits of yogurt consumption on health outcomes during paediatric age. Recent epidemiological studies evaluating the nutritional impact of yogurt have demonstrated its significant contribution to nutrients intakes among children. Thus, consuming yogurt is a strategy to achieve recommended nutrient intake and healthier dietary choices, with potential impact on obesity and cardiometabolic outcome in children. Yogurt's effects on paediatric infectious diseases, gastrointestinal diseases and atopic-related disorders are ascribed to the specific probiotic strain administered. Interestingly, the benefits of yogurt consumption are most likely due to effects mediated through the gut microbiota and the enhancement of innate and adaptive immune responses. Therefore, supplementing standard yogurt cultures with probiotic strains could be useful to promote health at different paediatric ages, although more evidence is needed regarding the strain-related effects and their interplay within the paediatric immune system.
Karwowska Z, Szemraj J, Karwowski BT. Anticancer properties of probiotic yogurt bacteria.. Postepy Biochem. 2019 Sep 30;65(3):163-172. Polish. doi: 10.18388/pb.2019_266.
Abstract. The intestinal microflora plays a key role in maintaining homeostasis in the human body. Microbes affect, among others, energy conversion and absorption of nutrients, regulate immune system and help to protect the host organism from pathogenic microorganisms. The balanced composition of the intestinal microflora can be easily disturbed and any changes caused by diet, stress, obesity, diseases of the digestive system or medication may lead to pro-inflammatory immune responses and initiation of disease processes, including cancer. Maintaining intestinal microflora homeostasis is therefore extremely important for human health. In order to restore it, it is most often used to take specimens with appropriate bacterial cultures, i. e. probiotics. Due to the fact that yoghurts are a source of probiotic bacteria, their regular consumption may be a strong point in the prevention of various types of diseases, including civilization diseases and cancer. This article reviews the literature in the area of using yogurt bacteria in the prevention of cancer. Issues addressed in the article relate to the characteristics of yogurt bacteria, beneficial effects of probiotics on human health, anti-cancer properties of yogurt bacteria and their metabolites, i. e. immunoregulation, prevention of bacterial infections, maintenance of cellular connections in the intestine and anti-cancer activity of bacterial metabolites.
Fernandez MA, Panahi S, Daniel N, Tremblay A, Marette A. Yogurt and Cardiometabolic Diseases: A Critical Review of Potential Mechanisms. Adv Nutr. 2017 Nov 15;8(6):812-829. doi: 10.3945/an.116.013946.
Abstract. Associations between yogurt intake and risk of diet-related cardiometabolic diseases (CMDs) have been the subject of recent research in epidemiologic nutrition. A healthy dietary pattern has been identified as a pillar for the prevention of weight gain and CMDs. Epidemiologic studies suggest that yogurt consumption is linked to healthy dietary patterns, lifestyles, and reduced risk of CMDs, particularly type 2 diabetes. However, to our knowledge, few to no randomized controlled trials have investigated yogurt intake in relation to cardiometabolic clinical outcomes. Furthermore, there has been little attempt to clarify the mechanisms that underlie the potential beneficial effects of yogurt consumption on CMDs. Yogurt is a nutrient-dense dairy food and has been suggested to reduce weight gain and prevent CMDs by contributing to intakes of protein, calcium, bioactive lipids, and several other micronutrients. In addition, fermentation with bacterial strains generates bioactive peptides, resulting in a potentially greater beneficial effect of yogurt on metabolic health than nonfermented dairy products such as milk. To date, there is little concrete evidence that the mechanisms proposed in observational studies to explain positive results of yogurt on CMDs or parameters are valid. Many proposed mechanisms are based on assumptions that commercial yogurts contain strain-specific probiotics, that viable yogurt cultures are present in adequate quantities, and that yogurt provides a minimum threshold dose of nutrients or bioactive components capable of exerting a physiologic effect. Therefore, the primary objective of this review is to investigate the plausibility of potential mechanisms commonly cited in the literature in order to shed light on the inverse associations reported between yogurt intake and various cardiometabolic health parameters that are related to its nutrient profile, bacterial constituents, and food matrix. This article reviews current gaps and challenges in identifying such mechanisms and provides a perspective on the research agenda to validate the proposed role of yogurt in protecting against CMDs. © 2017 American Society for Nutrition.
Wang X, Wang L, Wei X, Xu C, Cavender G, Lin W, Sun S. Invited review: Advances in yogurt development-Microbiological safety, quality, functionality, sensory evaluation, and consumer perceptions across different dairy and plant-based alternative sources. J Dairy Sci. 2025 Jan;108(1):33-58. doi: 10.3168/jds.2024-25322.
Abstract. Yogurt, as a globally prevalent fermented dairy product, is renowned for its substantial nutritional value and a myriad of health benefits, particularly pertaining to the digestive system. This narrative review elucidates the latest advancements in yogurt development from 2019 to 2024, addressing aspects of microbiological safety, quality, functionality, sensory evaluation, and consumer perceptions across diverse protein sources. The intrinsic quality of yogurt is notably influenced by its primary ingredient, milk, traditionally derived from animals such as cows, goats, and sheep. In recent years, plant-based yogurt (PBY) have emerged as a popular alternative to traditional dairy yogurts, that are made from plant sources and offer similar textures and flavors, catering to those seeking nondairy options. This discussion encompasses the advantages and limitations of various sources and explores methodologies to enhance yogurt quality using these diverse sources. Ensuring the microbiological safety of yogurt is thus paramount to its quality, as it involves both preventing the presence of harmful pathogens and managing spoilage to maintain freshness. This article encapsulates the potential hazards and corresponding antibacterial strategies that safeguard yogurt consumption. These strategies include the use of natural preservatives, advancements in packaging technologies, and the implementation of stringent hygiene practices throughout the production process. Moreover, the quality of yogurt is dependent not only on the source but also on the fermentation process and additional ingredients used. By addressing both the prevention of pathogen contamination and the control of spoilage organisms, this article explores comprehensive approaches but also examines the use of high-quality starter cultures, the role of prebiotics in enhancing probiotic efficacy, and genetic advancements, as well as improvements in the overall nutritional profile and shelf life of yogurt. Techniques to improve texture, flavor, and nutrient content are also discussed, providing a comprehensive overview of current quality enhancement methods. This analysis delves into the intricate mechanisms underpinning probiotic development, including the roles of prebiotics, supplementary starter cultures, and genetic factors that facilitate probiotic proliferation. These benefits include improved digestive health, enhanced immune function, and potential reductions in the risk of certain chronic diseases. Beyond quality and functionality, the sensory evaluation of yogurt remains crucial for consumer acceptance. In recent years, the incorporation of diverse additional ingredients into yogurt has been observed, aimed at augmenting its sensory attributes. This examination reveals these ingredients and their respective functions, such as natural flavorings, sweeteners, and texturizing agents, with the ultimate goal of enhancing overall consumer satisfaction. Consumer preferences exert a profound influence on yogurt production, rendering the understanding of customer opinions essential for devising competitive industry strategies. This article consolidates consumer feedback and preferences, striving to elevate yogurt quality and promote dietary diversity. The analysis includes trends such as the growing demand for organic and nondairy yogurts, the importance of sustainable practices, and the impact of marketing and packaging on consumer choices. This comprehensive overview serves as a valuable reference for the dairy industry and researchers dedicated to the advancement of yogurt development. The Authors. Published by Elsevier Inc. on behalf of the American Dairy Science Association®.
Whole milk yogurt 
