Leontopodium Alpinum Callus Extracellular Vesicles is a naturally derived compound.

Leontopodium Alpinum callus extracellular vesicles, derived from the culture of edelweiss cells, are a cutting-edge ingredient in the cosmetic and skincare industry. These vesicles are known for their regenerative, antioxidant, and protective properties, making them particularly suitable for anti-aging products and the care of sensitive or damaged skin.

Origin. Produced from the culture of Leontopodium Alpinum cells, a plant known for its resilience and ability to survive in extreme conditions.

Functions of Raw Materials.

Edelweiss Cells. These cells are cultivated in a laboratory setting and produce extracellular vesicles rich in bioactive compounds that promote skin regeneration and offer powerful antioxidant protection.

Industrial Production Process

• Cell Culture. Edelweiss cells are cultivated under controlled conditions to maximize the production of extracellular vesicles.
• Isolation. The extracellular vesicles are isolated from the cultivated cells.
• Purification. The vesicles are purified to remove impurities and concentrate the active compounds.
• Quality Control. Each batch of Leontopodium Alpinum callus extracellular vesicles undergoes rigorous quality checks to ensure their efficacy and safety.

What it is for and where

Cosmetics - INCI Functions

Skin conditioning agent - Miscellaneous.  This ingredient has the task of modifying and improving the condition of the skin when it is damaged or dry, reducing flaking and restoring its elasticity.

Form and Color

Leontopodium Alpinum callus extracellular vesicles appear as a clear and colorless liquid.

Leontopodium alpinum, known as Edelweiss, is an alpine plant that grows in high mountains with a distinctive flower. It is a rare plant and a protected species.

Phytochemical analysis found the presence of flavonoids and leontopodic acid and demonstrated potential DNA protective, antioxidant and antibacterial activity (1).

A recent study also demonstrated an anti-aging effect on the skin (2), and its extract (0.001%) is capable, used in a targeted manner directly at the site where it will be able to perform its efficacy, of inhibiting hair loss in female and male subjects by supporting hair follicle growth (3).

Protection Against Blue Light Damage. Research has shown that extracts from Leontopodium alpinum callus culture (LACCE) can be effective in protecting human skin cells from damage caused by blue light. This is particularly significant for skincare, as blue light from screens can contribute to skin aging and damage. The studies found that LACCE can promote collagen production, reduce harmful enzyme secretion, and decrease reactive oxygen species and calcium influx in skin cells. This suggests that LACCE could be a valuable ingredient in skincare products designed to protect against blue light (4).

Active Components and Mechanisms. Further investigation into the active components of LACCE identified several compounds, such as chlorogenic acid, isoquercitrin, and leontopodic acids, which contribute to its protective effects. These components were found to enhance skin cell viability and reduce damage markers when exposed to blue light. This research provides a deeper understanding of how LACCE works at a molecular level and supports its potential use in pharmaceutical and skincare products.

Cultivation and Biotechnological Production. Given that Leontopodium alpinum is a protected species in some European countries, there's interest in sustainable cultivation and biotechnological production methods. Studies have compared different cultivation techniques, including the use of gamma irradiation, to enhance the production of valuable compounds in the plant. This research is crucial for ensuring sustainable and ethical sourcing of Leontopodium alpinum for commercial use.

References_________________________________________________________________________

(1) Schwaiger, Stefan, et al. "Leontopodic acid—a novel highly substituted glucaric acid derivative from Edelweiss (Leontopodium alpinum Cass.) and its antioxidative and DNA protecting properties." Tetrahedron 61.19 (2005): 4621-4630.

Abstract. Leontopodic acid—a novel full substituted hexaric acid derivative, was isolated from the aerial parts of Edelweiss (Leontopodium alpinum Cass.) as one of the major compounds. The complex structure of leontopodic acid-2-[(3S)-3-hydroxybutanoate]-3,4,5-tris-[(2E)-3-(3,4-dihydroxyphenyl)-2-propenoate]-d-glucaric acid—was elucidated by mass spectrometry, 1D- and 2D NMR spectroscopy and HPLC monitored transesterification. Leontopodic acid exhibited pronounced antioxidative effects in the Briggs-Rauscher (BR) model [(r.a.c.)m 3.4±0.5] and the trolox equivalent antioxidant capacity (TEAC) method (TEAC value of 1.53±0.11). The antioxidative properties of this compound were confirmed by the 3D method, an in vitro assay evaluating DNA protection against oxidative damage (IC50: 1.89 μM).

(2) Cho WK, Kim HI, Kim SY, Seo HH, Song J, Kim J, Shin DS, Jo Y, Choi H, Lee JH, Moh SH. Anti-Aging Effects of Leontopodium alpinum (Edelweiss) Callus Culture Extract Through Transcriptome Profiling. Genes (Basel). 2020 Feb 21;11(2):230. doi: 10.3390/genes11020230. PMID: 32098197; PMCID: PMC7074254.

Abstract. Edelweiss (Leontopodium Alpinum) in the family Asteraceae is a wildflower that grows in rocky limestone places. Here, we investigated the efficacy of edelweiss callus culture extract (Leontopodium Alpinum callus culture extract; LACCE) using multiple assays from in vitro to in vivo as well as transcriptome profiling. Several in vitro assay results showed the strong antioxidant activity of LACCE in response to UVB treatment. Moreover, LACCE suppressed inflammation and wrinkling; however, moisturizing activity was increased by LACCE. The clinical test in vivo demonstrated that constant application of LACCE on the face and skin tissues improved anti-periorbital wrinkles, skin elasticity, dermal density, and skin thickness compared with the placebo. The RNA-Sequencing results showed at least 16.56% of human genes were expressed in keratinocyte cells. LACCE up-regulated genes encoding several KRT proteins; DDIT4, BNIP3, and IGFBP3 were involved in the positive regulation of the developmental process, programmed cell death, keratinization, and cornification forming skin barriers, which provide many advantages in the human skin. By contrast, down-regulated genes were stress-responsive genes, including metal, oxidation, wounding, hypoxia, and virus infection, suggesting LACCE did not cause any harmful stress on the skin. Our comprehensive study demonstrated LACCE is a promising agent for anti-aging cosmetics.

(3) Campiche R, Le Riche A, Edelkamp J, Botello AF, Martin E, Gempeler M, Bertolini M. An extract of Leontopodium alpinum inhibits catagen development ex vivo and increases hair density in vivo. Int J Cosmet Sci. 2022 Jun;44(3):363-376. doi: 10.1111/ics.12783. Epub 2022 Jun 6. PMID: 35514231; PMCID: PMC9328135.

Abstract. Objectives. Hair loss and reduction in hair volume are hallmarks of hair disorders, such as telogen effluvium, or male or female pattern hair loss, and hair ageing, which can cause severe distress in both men and women. Common anti‐hair loss drugs carry some side effects; therefore, novel, safer approaches targeting milder phenotypes are highly advocated. In this context, we investigated an extract of the alpine plant Edelweiss, Leontopodium alpinum var. Helvetia, for its ability to modulate hair follicle (HF) growth ex vivo and inhibit hair loss while increasing hair regeneration in vivo....Conclusions. We show here, through several lines of evidence, that the selected extract of the alpine plant Leontopodium alpinum var Helvetia (Edelweiss) inhibits premature catagen induction, possibly by stimulating dermal papilla inductivity. It is therefore worth exploiting this extract clinically as an anti‐hair loss agent, both for preventing ageing‐associated hair shedding and as an adjuvant therapy for hair loss disorders. Methods. Human amputated HFs were microdissected from three donors, two women and one man, and cultured ex vivo for 6 days. After treatment with 0.001% Edelweiss extract (EWDE), we investigated hair shaft production and anagen/catagen conversion, and measured known parameters associated with hair growth, that is hair matrix keratinocyte proliferation and apoptosis, dermal papilla inductivity, and growth factors, by quantitative (immuno)histomorphometry. To assess the anti‐hair loss potential of the alpine plant compound, we performed a randomized, placebo‐controlled human study enrolling Caucasian women and men, aged 18 to 65 years, with normal hair loss. After 5 months’ daily use of an extract containing leave‐on serum, we analysed hair density and anagen‐to‐catagen/telogen ratio by the Trichogram analysis....Conclusions. We show here, through several lines of evidence, that the selected extract of the alpine plant Leontopodium alpinum var Helvetia (Edelweiss) inhibits premature catagen induction, possibly by stimulating dermal papilla inductivity. It is therefore worth exploiting this extract clinically as an anti‐hair loss agent, both for preventing ageing‐associated hair shedding and as an adjuvant therapy for hair loss disorders.

(4) Li H, Meng X, Zhang Y, Guo M, Li L. Active Components of Leontopodium alpinum Callus Culture Extract for Blue Light Damage in Human Foreskin Fibroblasts. Molecules. 2023 Oct 28;28(21):7319. doi: 10.3390/molecules28217319.