Bruno HT è un composto chimico, un ingrediente inserito nella lista degli additivi alimentari europei come colorante azoico E155. Ha un colore marrone.

Sicurezza

Il problema legato ai coloranti azoici (monoazo o diazo) è la degradazione fotocatalitica che porta ad un'eventuale ossidazione ed alla successiva formazione di impurità come le ammine aromatiche alcune delle quali svolgono attività cancerogena.

Questo studio ritiene che, a seguito di dose giornaliera ripetuta,  nonostante la rapida eliminazione della maggior parte di una dose orale di Brown HT, alcuni metaboliti si accumulano nella maggior parte dei tessuti di animali da laboratorio (1).

Nessun effetto avverso è stato rilevato in questo studio a lungo termine (2) e nessun effetto avverso in questo altro studio su tre generazioni di ratti (3).

E' stata osservata una riduzione del colesterolo HDL e una diminuzione del peso dei ratti (4).

Bibliografia_____________________________________________________________________

(1) Phillips JC, Mendis D, Gaunt IF. Metabolic disposition of 14C-labelled Brown HT in the rat, mouse and guinea-pig. Food Chem Toxicol. 1987 Dec;25(12):1013-9. doi: 10.1016/0278-6915(87)90297-3.

Abstract. The absorption, metabolism, tissue distribution and excretion of 14C-labelled Brown HT has been studied in the rat, mouse and guinea-pig. Following administration of a single oral dose of either 50 or 250 mg Brown HT/kg, substantially all of the dose was excreted in the urine and faeces within 72 hr, with the majority (more than 80%) being accounted for in the faeces. A significant difference in urinary excretion of radioactivity was seen between male and female rats, as well as clear species differences at the two dose levels used. In all species studied, naphthionic acid was the major urinary metabolite, whereas in the faeces naphthionic acid, trace quantities of unchanged dye and at least two unidentified metabolites were found. Pregnant rats eliminated a single oral dose of 14C-labelled colouring at a rate similar to that in non-pregnant females, but some retention of radioactivity was found in the foetuses. Radioactivity was present in all tissues of male rats 24 hr after an oral dose of 250 mg 14C-labelled Brown HT/kg, with the highest concentrations in the gastro-intestinal tract, kidney and lymph nodes. Clearance from the gastro-intestinal tract was more rapid than from other tissues, but by day 7, the concentration of radioactivity (less than 0.001% of the dose/g) was similar in all tissues except the kidney and mesenteric lymph nodes. Similar results were obtained with animals pretreated for 21 days with either unlabelled or 14C-labelled Brown HT (250 mg/kg/day) prior to a radioactive dose. For most tissues examined, the concentration of radioactivity was greater with pretreatment than without. These results suggest that despite the rapid reduction and elimination of the major part of an oral dose of Brown HT, some colouring and/or metabolites accumulate in most tissues of male rats during repeated daily administration, but that only in the kidney and mesenteric lymph nodes is the accumulation tissue-specific. The accumulated radioactivity is cleared rapidly from most tissues on cessation of treatment. No significant absorption of either Brown HT, metabolites or subsidiary dyes was detected using isolated loops of small intestine.

(2) Carpanini FM, Butterworth KR, Gaunt IF, Kiss IS, Grasso P, Gangolli SD. Long-term toxicity studies on Chocolate Brown HT in rats. Toxicology. 1978 Nov;11(3):303-7. doi: 10.1016/s0300-483x(78)91839-5.

Abstract. Groups of 48 males and 48 female rats were given diets containing 0 (control), 500, 2000 or 10,000 ppm Chocolate Brown HT for 2 years. These treatments had no adverse effect on mortality, body-weight gain, food or water consumption, haematology, renal function, serum constituents, organ weight or histopathology. From the incidence of tumours observed in the control and test animals it is concluded that Chocolate Brown HT did not exert any carcinogenic effect and that the no-untoward-effect level was 10,000 ppm.

(3) Mangham BA, Moorhouse SR, Grant D, Brantom PG, Gaunt IF. Three-generation toxicity study of rats ingesting Brown HT in the diet. Food Chem Toxicol. 1987 Dec;25(12):999-1007. doi: 10.1016/0278-6915(87)90295-x.

Abstract. Brown HT was fed to rats of both sexes over three generations at dietary concentrations designed to provide daily intakes of 0, 50, 250 and 500 mg Brown HT/kg body weight/day. During the study a number of females died or failed to nurse their litters. This was so severe following the first mating of F1 adults that the animals were remated to provide the next generation. None of these effects were related to treatment. Body weight and food and water intakes were not adversely affected by treatment. No effects of treatment were seen on reproductive performance or foetal and pup development, apart from slight evidence of a treatment-related retarded ossification of the third sternebrae. Organ weights at autopsy showed two changes, one of which was increased kidney weights which, although not present in every generation, seemed to be related to treatment. The other, increased caecum weights, occurred in adult high-dose females of early generations, but not in males or later generations of the study. Apart from brown coloration of tissues, macroscopic and microscopic examination revealed no treatment-related changes. It was concluded that the no-untoward-effect level in the present study was 250 mg Brown HT/kg/day.

(4) Aboel-Zahab H, el-Khyat Z, Sidhom G, Awadallah R, Abdel-al W, Mahdy K. Physiological effects of some synthetic food colouring additives on rats. Boll Chim Farm. 1997 Nov;136(10):615-27.

Abstract. Three different synthetic chocolate colourant agents (A, B and C) were administered to healthy adult male albino rats for 30 and 60 day periods to evaluate their effects on body weight, blood picture, liver and kidney functions, blood glucose, serum and liver lipids, liver nucleic acids (DNA and RNA), thyroid hormones (T3 and T4) and growth hormone. In addition, histopathological examinations of liver, kidney and stomach sections were studied. These parameters were also investigated 30 days after colourant stoppage (post effect). Ingestion of colourant C (brown HT and indigocarmine) significantly decreased rat body weight, serum cholesterol and HDL-cholesterol fraction, while, T4 hormone, liver RNA content, liver enzymes (S. GOT, S. GPT and alkaline phosphatase), total protein and globulin fractions were significantly elevated. Significant increases were observed in serum total lipids, cholesterol, triglycerides, total protein, globulin and serum transaminases in rats whose diets were supplemented with chocolate colours A and B (sunset yellow, tartrazine, carmoisine and brilliant blue in varying concentrations). Haematological investigations demonstrated selective neutropenia and lymphocytosis with no significant alterations of total white blood cell counts in all rat groups, while haemoglobin concentrations and red blood cell counts were significantly decreased in the rats who were administered food additives A and B. Eosinophilia was noted in rats fed on colourant A only. No changes were recorded for blood glucose, growth hormone and kidney function tests. Histopathological studies showed brown pigment deposition in the portal tracts and Van Küpffer cells of the liver as well as in the interstitial tissue and renal tubular cells of the kidney mainly induced by colourant A. Congested blood vessels and areas of haemorrhage in both liver and renal sections were revealed in those rats who were given colourants B and C. There were no-untoward-effects recorded in the stomach tissue.