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Al222
Al222 (25264 pt) 2026-Aug-25 14:26

Lemon balm extract

Lemon balm (Melissa officinalis L.)

Lemon balm is a perennial aromatic species of the Lamiaceae family, cultivated for its leaves and flowering tops, with a citrus–herbaceous profile mainly due to oxygenated monoterpenes. It is used as a fresh or dried herb in herbal teas and foods, as a source of essential oil, and as a hydroalcoholic or glycolic extract to flavour beverages, confectionery products and culinary preparations.

Caloric value (leaves)
Fresh: approximately 30–45 kcal/100 g.
Dried: approximately 240–300 kcal/100 g (indicative value for dried herbs rich in fibre; the contribution in recipes is negligible at aromatic dosages).

Main substances contained
Total essential oil typically 0.05–0.3% on dry mass, with citronellal, citral (neral + geranial), geraniol, linalool and β-caryophyllene; traces of methyl citronellate.
Abundant phenolic fraction — rosmarinic acid predominant; caffeic derivatives — flavonoids, including luteolin, quercetin and apigenin glycosides, and triterpenes such as ursolic and oleanolic acids.
Fibre, chlorophylls and mineral salts — K, Ca, Mg — in the leaf matrix.
For extracts: carriers such as water, ethanol (EtOH) and glycerine.

Average composition (indicative, dried leaves, per 100 g)
Water: ~8–12 g.
Carbohydrates, mostly fibre: ~50–65 g.
Proteins: ~10–15 g.
Fats: ~3–6 g.
Ash: ~7–10 g.
aw — dried ingredient: low.

Production process
Cultivation and harvesting: perennial crops, harvested before full flowering, when the volatile profile is richer; multiple cuts per year in mild climates.
Drying and cleaning: gentle drying below 40 °C, in the shade and with ventilation to preserve volatiles and phenolics; stripping, sieving and removal of foreign bodies.
Standardisation of the dried herb: homogenisation by cutting or grinding; possible microbial load reduction; packaging in barrier packs.
Essential oil: steam distillation of fresh or dried leaves, separation, filtration and profile control by GC–MS.
Extracts: extraction in water/EtOH or glycerine at a defined drug/solvent ratio; filtration, mild concentration and titration of markers, for example rosmarinic acid, by HPLC.

Sensory and technological properties
Aroma: notes of fresh lemon, balsamic herbs and slight sweetness; the essential oil is intense but thermolabile.
Functionality: in herbal teas and extracts it provides a citrus and “green” tone; in pastry and ice cream it gives freshness and aromatic cleanliness; in beverages it can mask bitter notes.
Stability: whole leaves preserve aroma better than powder; light, heat and oxygen degrade both the essential oil and phenolics.
Solubility: the herb is water-soluble for the phenolic extract; volatile compounds require suitable carriers/alcohol and proper fixation.

Food uses
Herbal teas and infusions, syrups and flavoured drinks, traditional liqueurs and elixirs, sorbets and ice creams, ganaches and creams, salads and yogurt-based sauces, light marinades for fish and vegetables, flavoured sugars and salts. The essential oil is used in microdoses in compound flavourings.

Nutrition and health
The portions used are small and the energy impact is minimal. Lemon balm is traditionally associated with a “calming/digestive” perception; in food contexts, these effects should not be interpreted as health claims. The use of hydroalcoholic extracts must take into account the presence of EtOH on the label, where relevant.

Quality and specifications — typical issues
Botanical identity, controlled purity and moisture; absence of visible residues.
Essential oil content and terpene composition within range; rosmarinic acid content verified; consistent GC–MS/HPLC fingerprint.
For the oil: clear colour and absence of oxidised notes; for the herb: preserved green colour and clean aroma.
Traceability and compliance under GMP/HACCP, with CCP on harvesting, drying and packaging.

Storage and shelf life
Store in a cool, dry and dark place in tightly closed containers; monitor RH.
Prefer leaf herb for long storage and grind close to use; for the oil, use dark glass and minimum headspace.
Apply FIFO rotation.

Allergens and safety
Lamiaceae rarely cause cross-reactions, but individual sensitivities are possible. Powders may be irritating if inhaled during processing. The essential oil should be dosed according to good practice, avoiding overdosing in food.

INCI functions in cosmetics
Typical names: Melissa Officinalis Leaf Extract, Melissa Officinalis Oil, Melissa Officinalis Flower/Leaf/Stem Water. Functions: fragrance, masking agent, skin conditioning, perceived soothing action, supporting antioxidant in natural leave-on and rinse-off products.

Troubleshooting
Loss of aroma: drying temperature too high or exposure to light → Lower the temperature, protect from light/air, use whole leaves.
“Hay-like”/oxidised notes: long storage or non-barrier packaging → Improve the barrier, add moisture-absorbing sachets, shorten shelf life.
Instability in beverages: phenolic precipitation or volatility → Standardise the extract, use mild chelating agents and add cold.
Non-uniformity in doughs/mixtures: uneven distribution → Premix with sugars/salts or use a metered liquid extract.

Sustainability and supply chain
Low-input cultivation with soil and biodiversity management — including pollinating bees — improves the profile; green waste can be recovered as soil improver/biomass. Managing processing effluents towards BOD/COD targets, recyclable packaging and efficient sanitation reduce the environmental impact.

Conclusion
Lemon balm offers a distinctive and versatile citrus–herbaceous profile, effective at low dosage in herbal teas, beverages and desserts. Its application quality depends on maturity, gentle drying, protection from light/oxygen and correct standardisation of extracts; with these controls, sensorially stable and repeatable products can be obtained.

Mini-glossary
aw — Water activity: fraction of “free” water; low aw supports the stability of dried herbs.
RH — Relative humidity: ambient humidity; high values accelerate aroma loss and caking.
EtOH — Ethanol: hydroalcoholic carrier for extracts; to be considered for suitability and declarations.
GC–MS — Gas chromatography–mass spectrometry: fingerprint of the essential oil and quality control.
HPLC — High-performance liquid chromatography: titration of markers, for example rosmarinic acid, in extracts.
GMP — Good manufacturing practice: good practices for hygiene, consistency and traceability.
HACCP — Hazard analysis and critical control points: preventive system with definition of CCP.
CCP — Critical control point: a step where control prevents, eliminates or reduces a hazard to acceptable levels.
FIFO — First in, first out: stock rotation requiring the oldest batches to be used first.
INCI — International Nomenclature of Cosmetic Ingredients: standard nomenclature for cosmetic ingredients.
BOD/COD — Biochemical/chemical oxygen demand: indicators of the organic load of effluents and environmental impact.

Studies

It is among the oldest and most common medicinal plants and yields an essential oil with sedative, antispasmodic, carminative, antibacterial, antiviral, anti-inflammatory, antioxidant and neuroprotective effects (1).

It contains components of considerable interest for human health. The results of this study indicate that Melissa officinalis could be considered an effective agent in the prevention of various neurological conditions associated with oxidative stress: quercetin showed the highest antioxidant activity, followed by gallic acid, quercitrin and rutin. It also contains hydroxycinnamic acid, m-coumaric acid, caffeic acid (2), alpha-tocopherol and flavonoids (3).

Solar ultraviolet radiation is one of the main causes of several skin disorders, including photoageing and skin cancer. Its UVB component — 280–315 nm — leads to oxidative stress and causes inflammation, DNA damage, p53 induction and lipid and protein oxidation. Recently, the use of plant polyphenols with antioxidant and anti-inflammatory properties to protect human skin against the harmful effects of sunlight has been increasing. This study positively evaluates the protective effects of Melissa officinalis extract and its main phenolic compound, rosmarinic acid, against UVB-induced damage in human keratinocytes (4).

Age-related macular degeneration is one of the most common causes of irreversible vision loss among elderly people in developed countries. Melissa officinalis extract protects human retinal pigment epithelial cells against apoptosis induced by oxidative stress (5).

Insomnia. An interesting study starts from the premise that some of the most widely used drugs for insomnia include benzodiazepines, barbiturates, some antidepressants and some first-generation antihistamines. Drug dependence or drowsiness is evident among the adverse effects of these drugs. Although most antihistamines have sedative effects, anticholinergic complications and other side effects limit their use. This study found that extracts of Lavandula angustifolia and Melissa officinalis showed additive effects and suggests that a preparation containing both extracts may be useful for insomnia (6).

Cosmetics

Skin conditioning agent - Mixed. This ingredient has the function of modifying the condition of the skin when it is damaged or dry, reducing flaking and restoring elasticity.

Melissa studies

Bibliography________________________________________________________________________

(1) Kamdem JP, Adeniran A, Boligon AA, Klimaczewski CV, Elekofehinti OO, Hassan W, et al. Antioxidant activity, genotoxicity and cytotoxicity evaluation of lemon balm (Melissa officinalis L.) ethanolic extract: its potential role in neuroprotection. Ind Crops Prod. 2013;51:26–34.

Abstract. The antioxidant activity of Melissa officinalis (MO) was evaluated to understand the mechanism of its pharmacological properties as well as its potential genotoxic and cytotoxic effects in human leukocytes. The results showed that MO scavenged DPPH radical in a concentration dependent-manner with IC50 values of 48.76 ± 1.94 μg/mL. MO showed strong reducing power and exhibited a significant inhibition of deoxyribose degradation. MO interfered with the formation of 1,10-phenanthroline–Fe2+ complex, suggesting that it has chelating activity and captures Fe2+ before 1,10-phenanthroline. The addition of 5 mM ascorbic acid to the reaction mixture dramatically reduced Fe3+ (formed during the incubation time) to Fe2+ indicating that it was an “apparent” chelation. MO was neither genotoxic nor cytotoxic at the concentrations tested, indicating that the popular use of the extract might possibly not result in any genotoxic or cytotoxic effects. Our results suggest that MO is a potential source of natural antioxidants, and could be relevant for the management of oxidative stress. Of particular importance, for neurodegenerative diseases, the capacity of MO to “chelate” and to maintain Fe2+ in a Fe3+ state can contribute to its neurotherapeutic effects, because iron plays a central role in brain damage.

(2) Pereira RP, Fachinetto R, de Souza Prestes A, Puntel RL, da Silva GN, Heinzmann BM, et al. Antioxidant effects of different extracts from Melissa officinalis, Matricaria recutita and Cymbopogon citratus. Neurochem Res. 2009;34(5):973–983

Abstract. Considering the important role of oxidative stress in the pathogenesis of several neurological diseases, and the growing evidence of the presence of compounds with antioxidant properties in the plant extracts, the aim of the present study was to investigate the antioxidant capacity of three plants used in Brazil to treat neurological disorders: Melissa officinalis, Matricaria recutita and Cymbopogon citratus. The antioxidant effect of phenolic compounds commonly found in plant extracts, namely, quercetin, gallic acid, quercitrin and rutin was also examined for comparative purposes. Cerebral lipid peroxidation (assessed by TBARS) was induced by iron sulfate (10 microM), sodium nitroprusside (5 microM) or 3-nitropropionic acid (2 mM). Free radical scavenger properties and the chemical composition of plant extracts were assessed by 1'-1' Diphenyl-2' picrylhydrazyl (DPPH) method and by Thin Layer Chromatography (TLC), respectively. M. officinalis aqueous extract caused the highest decrease in TBARS production induced by all tested pro-oxidants. In the DPPH assay, M. officinalis presented also the best antioxidant effect, but, in this case, the antioxidant potencies were similar for the aqueous, methanolic and ethanolic extracts. Among the purified compounds, quercetin had the highest antioxidant activity followed by gallic acid, quercitrin and rutin. In this work, we have demonstrated that the plant extracts could protect against oxidative damage induced by various pro-oxidant agents that induce lipid peroxidation by different process. Thus, plant extracts could inhibit the generation of early chemical reactive species that subsequently initiate lipid peroxidation or, alternatively, they could block a common final pathway in the process of polyunsaturated fatty acids peroxidation. Our study indicates that M. officinalis could be considered an effective agent in the prevention of various neurological diseases associated with oxidative stress.

(3) Koksal E, Bursal E, Dikici E, Tozoglu F, Gulcin I. Antioxidant activity of Melissa officinalis leaves. J Med Plant Res. 2011;5(2):217–22.

Abstract.The purpose of this study was to evaluate antioxidant activities of water extract of Melissa officinalis (WEM) and ethanol extract of M. officinalis (EEM), comparatively. The WEM and EEM were evaluated for their radical scavenging activities by means of the DPPH and DMPD assays. WEM scavenged radicals effectively with IC50 values of 31.4 µg/mL for DPPH free radical and 60.5 µg /mL for DMPD cation radical. Similarly, EEM scavenged radicals effectively with IC50 values of 202.7 µg/mL for DPPH free radical and 120.9 µg/mL for DMPD cation radical. Also, total reducing power of WEM was found higher than EEM with both potassium ferricyanide reduction (FRAP) and cupric ions reduction capacity methods (CUPRAC). The present study showed that WEM have effective antioxidant and radical scavenging activities as compared to EEM. 

(4) Pérez-Sánchez A, Barrajón-Catalán E, Herranz-López M, Castillo J, Micol V. Lemon balm extract (Melissa officinalis, L.) promotes melanogenesis and prevents UVB-induced oxidative stress and DNA damage in a skin cell model. J Dermatol Sci. 2016 Nov;84(2):169-177. doi: 10.1016/j.jdermsci.2016.08.004. 

(5) Jeung IC, Jee D, Rho CR, Kang S. Melissa Officinalis L. Extracts Protect Human Retinal Pigment Epithelial Cells against Oxidative Stress-Induced Apoptosis. Int J Med Sci. 2016 Feb 3;13(2):139-46. doi: 10.7150/ijms.13861.

Abstract. Background: We evaluated the protective effect of ALS-L1023, an extract of Melissa officinalis L. (Labiatae; lemon balm) against oxidative stress-induced apoptosis in human retinal pigment epithelial cells (ARPE-19 cells). Methods: ARPE-19 cells were incubated with ALS-L1023 for 24 h and then treated with hydrogen peroxide (H2O2). Oxidative stress-induced apoptosis and intracellular generation of reactive oxygen species (ROS) were assessed by flow cytometry. Caspase-3/7 activation and cleaved poly ADP-ribose polymerase (PARP) were measured to investigate the protective role of ALS-L1023 against apoptosis. The protective effect of ALS-L1023 against oxidative stress through activation of the phosphatidylinositol 3-kinase/protein kinase B (PI3K/Akt) was evaluated by Western blot analysis. Results: ALS-L1023 clearly reduced H2O2-induced cell apoptosis and intracellular production of ROS. H2O2-induced oxidative stress increased caspase-3/7 activity and apoptotic PARP cleavage, which were significantly inhibited by ALS-L1023. Activation of the PI3K/Akt pathway was associated with the protective effect of ALS-L1023 on ARPE-19 cells. Conclusions: ALS-L1023 protected human RPE cells against oxidative damage. This suggests that ALS-L1023 has therapeutic potential for the prevention of dry age-related macular degeneration.

(6) Hajhashemi V, Safaei A. Hypnotic effect of Coriandrum sativum, Ziziphus jujuba, Lavandula angustifolia and Melissa officinalis extracts in mice. Res Pharm Sci. 2015 Nov-Dec;10(6):477-84.

Abstract. The aim of the present study was to evaluate hypnotic effect of Coriandrum sativum, Ziziphus jujuba, Lavandula angustifolia and Melissa officinalis hydroalcoholic extracts in mice to select the most effective ones for a combination formula. Three doses of the extracts (250, 500 and 1000 mg/kg of C. sativum and Z. jujuba and 200, 400 and 800 mg/kg of L. angustifolia and M. officinalis) were orally administered to male Swiss mice (20-25 g) and one hour later pentobarbital (50 mg/kg, i.p.) was injected to induce sleep. Onset of sleep and its duration were measured and compared. Control animals and reference group received vehicle (10 ml/kg, p.o.) and diazepam (3 mg/kg, i.p.), respectively. C. sativum and Z. jujuba failed to change sleep parameters. L. angustifolia at doses of 200, 400 and 800 mg/kg shortened sleep onset by 7.6%, 50% and 51.5% and prolonged sleep duration by 9.9%, 43.1% and 80.2%, respectively. Compared with control group the same doses of M. officinalis also decreased sleep onset by 24.7%, 27.5% and 51.2% and prolonged sleep duration by 37.9%, 68.7% and 131.7% respectively. Combinations of L. angustifolia and M. officinalis extracts showed additive effect and it is suggested that a preparation containing both extracts may be useful for insomnia.