Description

r-Clostridium Histolyticum Collagenase H is a single-chain recombinant Clostridium histolyticum protein, with one mutation, produced by fermentation in E. coli. The starting gene is directly isolated from Clostridium histolyticum, which codes for the enzyme collagenase.

Chemical Composition and Structure

The chemical composition of r-Clostridium Histolyticum Collagenase H includes a specific sequence of amino acids that constitute the collagenase protein, with a single mutation. This protein is designed to maintain the enzymatic activity specific to collagenase.

Physical Properties

r-Clostridium Histolyticum Collagenase H typically appears as a clear to slightly opalescent liquid or as a fine powder when lyophilized. It is soluble in water and appropriate buffers, making it easily incorporable into various formulations.

Production Process

The production of r-Clostridium Histolyticum Collagenase H involves several steps:

Gene Isolation: The gene encoding for collagenase is directly isolated from Clostridium histolyticum.
Mutation: The isolated gene is modified to introduce a specific mutation.
Fermentation: The modified gene is introduced into E. coli, which is then fermented under controlled conditions to produce the recombinant protein.
Extraction: The protein is extracted from the E. coli cells using appropriate methods.
Purification: The extracted protein undergoes purification processes to remove any impurities and ensure high purity.
Lyophilization (if producing a powder): The purified protein is lyophilized to produce a fine powder.
Packaging: The final product is packaged under controlled conditions to ensure stability and purity.

Applications

Cosmetics: In the cosmetics industry, r-Clostridium Histolyticum Collagenase H is valued for its potential skin remodeling and texture-improving properties. It is commonly included in formulations of creams, serums, and lotions to enhance the appearance of the skin.
Medical and Pharmaceutical: This recombinant protein is also used in various medical and pharmaceutical applications for its enzymatic activity and potential therapeutic benefits.
Environmental and Safety Considerations

Environmental Impact: The production of r-Clostridium Histolyticum Collagenase H generally has a moderate environmental impact. Sustainable production practices and the use of eco-friendly methods can help minimize the overall impact.
Safety: r-Clostridium Histolyticum Collagenase H is considered safe for topical use in the concentrations typically employed in consumer products. However, it is essential to ensure that the protein is free from contaminants and complies with regulatory standards. Allergic reactions are rare but cannot be excluded.

INCI


Skin conditioning agent. It is the mainstay of topical skin treatment as it has the function of restoring, increasing or improving skin tolerance to external factors, including melanocyte tolerance. The most important function of the conditioning agent is to prevent skin dehydration, but the subject is rather complex and involves emollients and humectants that can be added in the formulation.
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